Cryopreservation of Beluga (Huso huso) Sperm: Effect of Different C
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چکیده
Semen obtained from four Beluga males (Huso huso) was cryopreserved using extender; Tris-sucroseKCl (30mM Tris, 23.4mM sucrose, 0.25mM KCl, PH 8.0) supplemented with MeOH at concentration of 5%, 10% and 20%. Semen was diluted, respectively, with ratios of 1:0.5, 1:1 and 1:2 with extender and frozen in liquid nitrogen vapor.Frozen sperms after 30 and 60 days were excluded from freezing. Experiment showed the highest motility duration and the most motility percentage of post-thawed sperms after 30 days was relatedto the treatments with the concentration of MeOH10% and the dilution ratio of 1:1 (157±25.46 s and 12.58±4.94%; P<0.05), as well as the upmost mobility and the motility duration of post-thawed sperms after 60 days was related to the treatments with the concentration of MeOH10% and the dilution ratio of 1:1 (119.18±23.52 s and 10.31±4.12%; P<0.05).
منابع مشابه
Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa.
Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10°C, -15°C, -20°C, -30°C and -40°C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/1...
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